We develop and share genetic technologies, model systems, and analytical resources for studying neural development and brain cancer.
Addgene plasmids
Selected plasmids deposited by the Breunig Lab. Follow the Addgene links for maps, sequences, availability, and ordering details.
Recombinase tools
MADR reporters and donor constructs
- MADR pDonor TagBFP2-3XFlag (cyto) WPRE — Addgene #129421
- MADR pDonor smFP-FLAG (dark) WPRE — Addgene #131005
- MADR pDonor smFP-V5 (dark) WPRE — Addgene #131006
- MADR pDonor smFP-HA (dark) WPRE — Addgene #131007
- MADR pDonor smFP-Myc (dark) WPRE — Addgene #130987
Oncogenic and brain tumor model donors
- MADR pDonor TagBFP2-V5-HRAS G12V WPRE — Addgene #129420
- MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE — Addgene #131730
- MADR H3F3A-WT / PDGFRA D842V / TRP53 donor — Addgene #131461
- MADR H3F3A-K27M / PDGFRA D842V / TRP53 donor — Addgene #131462
- MADR H3F3A-G34R / PDGFRA D842V / TRP53 donor — Addgene #131463
Data & genomics
Public sequencing datasets deposited in the NIH Gene Expression Omnibus (GEO).
- GSE131675 — MADR study — GEO SuperSeries
- GSE131873 — MADR study — single-cell ATAC-seq
- GSE131672 — MADR study — ChIP-seq
- GSE346465 — Published sequencing dataset
- GSE341165 — Published sequencing dataset
Protocols & methods publications
Step-by-step methods for somatic genome engineering, brain tumor modeling, electroporation, and neural progenitor systems.
- Tetracycline-Inducible and Reversible Stable Gene Expression in Human iPSC-Derived Neural Progenitors and in the Postnatal Mouse Brain — Current Protocols (2023)
- Murine-Derived Glioma Organoids and Cell Line Culture Systems — Current Protocols (2023)
- Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures — JoVE (2023)
- Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR) — STAR Protocols (2020)
- Tetracycline-Inducible and Reversible Stable Gene Expression in Human iPSC-Derived Neural Progenitors and in the Postnatal Mouse Brain — Current Protocols in Stem Cell Biology (2017)
- Neonatal Pial Surface Electroporation — JoVE (2014)
Model systems
Contact the lab to discuss published model systems and available materials.